Impact of Plant Growth regulators (PGRs) on callus induction from internodal explants of Tecomella undulata (Sm.) Seem- A Multipurpose Medicinal plants

A study of callus induction of Tecomella undulata was conducted using a various concentrations of PGRs namely cytokinins such as BAP and KIN and auxins such as 2, 4-D, NAA and IAA. Internodal explants were aseptically cultured on MS medium supplemented with different concentrations (1.03.0 mg/l) of BAP, KIN, 2, 4-D, IAA and NAA. After two weeks callus was formed at the cut surface of the explants. The highest number of callus forming was obtained from explants cultured on the medium supplemented with 2.0 mg/l in 2.5mg/l 2,4-D alone were the induced calli were ceramish white in colour and structurally friable. Abbreviations sMS Murashige and Skoog (1962) basal medium; BAP Benzyl amino purine; KINKinetin 2,4-D 2,4Dichlorophenoxyacetic acid; NAA a-naphthalene acetic acid; IAA Indole acetic acid.